Preface

This introduction to base R course was designed for biologists with no previous programming experience. It has a focus on analysing RNA-sequencing data using the Bioconductor package limma. The F1000 paper ‘RNA-seq analysis is easy as 1-2-3 with limma, Glimma and edgeR’ was used as a guide for the latter sessions.

It is intended to be delivered over four 2.5 hour workshops at the Walter and Eliza Hall Institute of Medical Research (WEHI).

0.1 Data files

The data files require for this workshop are available on Github. To download a data file click on the file you wish to download then right click ‘Raw’ > ‘Save Link As…’:

Acknowledgements

Thank you to Shian Su, Brendan Ansell and Professor Gordon Smyth for your suggestions and review of this tutorial.
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